Concentration of hCG in Blastocoel Fluid is Independent of Embryo Morphology and PGT-A Results: Could it be a Significant Biomarker for a Competent Embryo?

The aim of this research was to develop a quantitative sandwich-strip ELISA developed on nitrocellulose membrane with anti-H alpha and anti-hCG beta antibodies. detection antibody conjugated horseradish peroxidase, luminol substrate following optimization hCG. Concentration hCG in the BF determined for 101 embryo biopsies (Study 1) 96 2) respectively, results were compared previously recorded morphology PGT-A results. present 73.6% all specimens analysed these two studies, weak correlation between levels both outcome observed. Additionally, receiver operating characteristic (ROC) curves show separately that do not correlate concentrations. This indicates presence produced by is separate, independent marker quality.